abstract：:Human immunodeficiency virus (HIV)-infected cells actively release the transcriptional activator (Tat) viral protein that is required for efficient HIV gene transcription. Extracellular Tat is able to enter uninfected cells. We recently reported that internalized Tat escapes endosomes to reach the cytosol and is then ...

journal_title：Biochimie

authors： Beaumelle B,Tóth P,Malak OA,Chopard C,Loussouarn G,Vitale N

更新日期：2017-10-01 00:00:00

abstract：PURPOSE:We evaluated the clinical relevance of microRNA-149 (miR-149) in neuroblastoma (NB) and its functional roles in regulating NB proliferation in vitro. METHODS:QRT-PCR was used to evaluate miR-149 expression in NB cell lines and primary NB tumors. Association between endogenous miR-149 expression in primary NB t...

journal_title：Biochimie

authors： Xu Y,Chen X,Lin L,Chen H,Yu S,Li D

更新日期：2017-08-01 00:00:00

abstract：:A reliable phylogenetic reconstruction of the evolutionary history of contemporary species depends on a robust identification of the universal common ancestor (UCA) at the root of the Tree of Life (ToL). That root polarizes the tree so that the evolutionary succession of ancestors to descendants is discernable. In eff...

journal_title：Biochimie

authors： Harish A,Kurland CG

更新日期：2017-07-01 00:00:00

abstract：:Inducing brown adipocytes in white adipose tissues is a promising target to combat obesity and its related disorders in human beings. This goal has been especially encouraged by new important discoveries of human brown adipose tissues. The accumulating evidence confirms the presence of active brown adipocytes, not onl...

journal_title：Biochimie

authors： Chu DT,Gawronska-Kozak B

更新日期：2017-07-01 00:00:00

abstract：:Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake (Micrurus spp.) venoms, and their role in envenomation remains largely unknown. In this report, we d...

journal_title：Biochimie

authors： Rey-Suárez P,Núñez V,Saldarriaga-Córdoba M,Lomonte B

更新日期：2017-06-01 00:00:00

abstract：:RNA domain swapping typically demonstrates conservation of the native function of the domain in a non-native context. In contrast, we employed RNA engineering to demonstrate deviation of G-quadruplex (GQ) function that is contingent upon its context dependent location, which is opposite to their native functional role...

journal_title：Biochimie

authors： Bhattacharyya D,Morris MJ,Kharel P,Mirihana Arachchilage G,Fedeli KM,Basu S

更新日期：2017-06-01 00:00:00

abstract：:Over the last couple of decades, the PPAR family of transcription factors has received much attention from the pharmaceutical industry due to their profound ability to improve glucose and lipid metabolism upon agonist activation. However, more recently the interest in these nuclear receptors has faded because several ...

journal_title：Biochimie

authors： Bugge A,Holst D

更新日期：2017-05-01 00:00:00

abstract：:The variable manifestation of phenotypes that occur in patients with neurofibromatosis type 1 (NF1) includes benign and malignant neurocutaneous tumors for which no adequate treatment exists. Cell-based screening of known bioactive compounds library identified the protein phosphatase 2A (PP2A) inhibitor Cantharidin an...

journal_title：Biochimie

authors： Semenova G,Stepanova DS,Deyev SM,Chernoff J

更新日期：2017-04-01 00:00:00

abstract：:Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiologic...

journal_title：Biochimie

authors： Ryuzono S,Takase R,Kamada Y,Ikenaga T,Chigwechokha PK,Komatsu M,Shiozaki K

更新日期：2017-04-01 00:00:00

abstract：:The possibility that brown adipose tissue thermogenesis can be recruited in order to combat the development of obesity has led to a high interest in the identification of "browning agents", i.e. agents that increase the amount and activity of UCP1 in brown and brite/beige adipose tissues. However, functional analysis ...

journal_title：Biochimie

authors： Kalinovich AV,de Jong JM,Cannon B,Nedergaard J

更新日期：2017-03-01 00:00:00

abstract：:We are here reporting that calpain-1 is a constitutive component of a distinct lipid raft/caveolin-1 microdomain isolated from bEnd5 cells in association with endothelial nitric oxide synthase (eNOS) and heat shock protein 90 (HSP90). Perturbations in intracellular calcium concentration by Ca2+-ionophore A23187 or pro...

journal_title：Biochimie

authors： Martines A,Stifanese R,Faelli EL,Perasso L,Melloni I,Ruggeri P,Averna M

更新日期：2017-02-01 00:00:00

abstract：:The microbial resistance to antibiotics is a genuine global threat. Consequently, a search of new inhibitors remains of acute importance due to the increasing spread of multidrug resistance. Here we present a new type of non-β-lactam β-lactamase inhibitor PA-34 based on natural phenoxyaniline, identified using compute...

journal_title：Biochimie

authors： Grigorenko VG,Andreeva IP,Rubtsova MY,Deygen IM,Antipin RL,Majouga AG,Egorov AM,Beshnova DA,Kallio J,Hackenberg C,Lamzin VS

更新日期：2017-01-01 00:00:00

abstract：:Aptamers are short single-stranded nucleic acid molecules that are selected in vitro from a large random sequence library based on their high and specific affinity to a target molecule by a process known as SELEX. Cell-SELEX that employs whole living cells overexpressing the defined cell surface proteins (for selectio...

journal_title：Biochimie

authors： Takahashi M,Sakota E,Nakamura Y

更新日期：2016-12-01 00:00:00

abstract：:The insomniac protein of Drosophila melanogaster (INC) has a crucial role in sleep homeostasis as flies lacking the inc gene exhibit strikingly reduced and poorly consolidated sleep. Nevertheless, in vitro characterizations of INC biophysical properties and partnerships have not been yet reported. Here we report the h...

journal_title：Biochimie

authors： Pirone L,Smaldone G,Esposito C,Balasco N,Petoukhov MV,Spilotros A,Svergun DI,Di Gaetano S,Vitagliano L,Pedone EM

更新日期：2016-12-01 00:00:00

abstract：:Kex2 is a Ca2+-dependent serine protease from S. cerevisiae. Characterization of the substrate specificity of Kex2 is of particular interest because this protease serves as the prototype of a large family of eukaryotic subtilisin-related proprotein-processing proteases that cleave sites consisting of pairs or clusters...

journal_title：Biochimie

authors： Manfredi MA,Antunes AA,Jesus LO,Juliano MA,Juliano L,Judice WA

更新日期：2016-12-01 00:00:00

abstract：:Long non-coding RNAs constitute the most abundant part of the transcribed mammalian genome. lncRNAs affect all essential processes in the living cell including transcription, splicing, translation, replication, shaping of chromatin and post translational modification of proteins. Alterations in lncRNA expression have ...

journal_title：Biochimie

authors： Smekalova EM,Kotelevtsev YV,Leboeuf D,Shcherbinina EY,Fefilova AS,Zatsepin TS,Koteliansky V

更新日期：2016-12-01 00:00:00

abstract：:Lipids are unevenly distributed within eukaryotic cells, allowing various processes to work in an optimized membrane environment. Rather than following vesicular trafficking pathways, certain lipids are transported by lipid transfer proteins (LTPs), some at sites of close apposition of two organelles called membrane c...

journal_title：Biochimie

authors： Moser von Filseck J,Drin G

更新日期：2016-11-01 00:00:00

abstract：:Glycosphingolipids and sphingolipids of cellular plasma membranes (PMs) reach luminal intra-lysosomal vesicles (LVs) for degradation mainly by pathways of endocytosis. After a sorting and maturation process (e.g. degradation of sphingomyelin (SM) and secretion of cholesterol), sphingolipids of the LVs are digested by ...

journal_title：Biochimie

authors： Sandhoff K

更新日期：2016-11-01 00:00:00

abstract：:Glycolipids constitute a class of molecules with various biological activities. Among them, sugar-based bolaamphiphiles characterized by their biocompatibility, biodegradability and lower toxicity, became interesting for the development of efficient and low cost lipid-based drug delivery systems. Their activity seems ...

journal_title：Biochimie

authors： Nasir MN,Crowet JM,Lins L,Obounou Akong F,Haudrechy A,Bouquillon S,Deleu M

更新日期：2016-11-01 00:00:00

abstract：:We have proposed an allosteric ATP inhibition mechanism of Pfk-2 determining the structure of different forms of the enzyme together with a kinetic enzyme analysis. Here we complement the mechanism by using hybrid oligomers of the homodimeric enzyme to get insights about the allosteric communication pathways between t...

journal_title：Biochimie

authors： Villalobos P,Soto F,Baez M,Babul J

更新日期：2016-09-01 00:00:00

abstract：:Skin aging is characterized by different features including wrinkling, atrophy of the dermis and loss of elasticity associated with damage to the extracellular matrix protein elastin. The aim of this study was to investigate the aging process of skin elastin at the molecular level by evaluating the influence of intrin...

journal_title：Biochimie

authors： Mora Huertas AC,Schmelzer CE,Hoehenwarter W,Heyroth F,Heinz A

更新日期：2016-09-01 00:00:00

abstract：:Snake venom Kunitz-type serine protease inhibitors (KSPIs) exhibit various biological functions including anticoagulant activity. This study elucidates the occurrence and subunit stoichiometry of a putative complex formed between two KSPIs (Rusvikunin and Rusvikunin-II) purified from the native Rusvikunin complex of P...

journal_title：Biochimie

authors： Mukherjee AK,Dutta S,Kalita B,Jha DK,Deb P,Mackessy SP

更新日期：2016-09-01 00:00:00

abstract：:Calcineurin (CN) is a unique calcium/calmodulin (CaM)-activated serine/threonine phosphatase. To perform its diverse biological functions, CN communicates with many substrates and other proteins. In the physiological activation of T cells, CN acts through transcriptional factors belonging to the NFAT family and other ...

journal_title：Biochimie

authors： Zhao Y,Zhang J,Shi X,Li J,Wang R,Song R,Wei Q,Cai H,Luo J

更新日期：2016-08-01 00:00:00

abstract：:The systems involving folate and cobalamin have several features in common: 1) their dietary forms require luminal digestion for absorption; 2) intestinal bacteria in the upper intestine synthesize and utilize both vitamins, creating possible competition for the nutrients; 3) there is one major intestinal brush border...

journal_title：Biochimie

authors： Alpers DH

更新日期：2016-07-01 00:00:00

abstract：:The recent increase in the intake of folic acid by the general public through fortified foods and supplements, has raised safety concern based on early reports of adverse health outcome in elderly with low B12 status who took high doses of folic acid. These safety concerns are contrary to the 2015 WHO statement that "...

journal_title：Biochimie

authors： Selhub J,Rosenberg IH

更新日期：2016-07-01 00:00:00

abstract：:Homocysteine (HCY) is a degradation product of the methionine pathway. The B vitamins, in particular vitamin B12 and folate, are the primary nutritional determinant of HCY levels and therefore their deficiencies result in hyperhomocysteinaemia (HHCY). Prevalence of hyperhomocysteinemia (HHCY) and related dietary defic...

journal_title：Biochimie

authors： Feigerlova E,Demarquet L,Guéant JL

更新日期：2016-07-01 00:00:00

abstract：:Plant serine proteases have been widely used in food science and technology as well as in medicine. In this sense, several plant serine proteases have been proposed as potential anti-coagulants and anti-platelet agents. Previously, we have reported the purification and identification of a plant serine protease from So...

journal_title：Biochimie

authors： Pepe A,Frey ME,Muñoz F,Fernández MB,Pedraza A,Galbán G,García DN,Daleo GR,Guevara MG

更新日期：2016-06-01 00:00:00

abstract：:The field of immunometabolism has come a long way in the past decade, leading to the emergence of a new role for white adipose tissue (WAT) that is now recognized to stand at the junction of immune and metabolic regulations. Interestingly, a crucial role of the abundant and heterogeneous immune population present in W...

journal_title：Biochimie

authors： Cousin B,Casteilla L,Laharrague P,Luche E,Lorsignol A,Cuminetti V,Paupert J

更新日期：2016-05-01 00:00:00

abstract：:Obesity prevalence continues to rise throughout the developed world, as a result of positive energy balance and reduced physical activity. At present, there is still a perception within the general community, and amongst some nutritionists, that eating multiple small meals spaced throughout the day is beneficial for w...

journal_title：Biochimie

authors： Hutchison AT,Heilbronn LK

更新日期：2016-05-01 00:00:00

abstract：:Cysteine-rich secretory proteins (CRISPs), characterized by 16 conserved cysteines, are distributed in a wide range of organisms, such as secernenteas, amphibians, reptiles and mammals. In the previous studies, a novel CRISP family member (cysteine-rich buccal gland protein, CRBGP) was separated from the buccal gland ...

journal_title：Biochimie

authors： Jiang Q,Liu Y,Duan D,Gou M,Wang H,Wang J,Li Q,Xiao R

更新日期：2016-04-01 00:00:00

abstract：:Chagasin-type inhibitors comprise natural inhibitors of papain-like cysteine proteases that are distributed among Protist, Bacteria and Archaea. Chagasin was identified in the pathogenic protozoa Trypanosoma cruzi as an approximately 11 kDa protein that is a tight-binding and highly thermostable inhibitor of papain, c...

journal_title：Biochimie

authors： Costa TF,Lima AP

更新日期：2016-03-01 00:00:00

abstract：:Mammalian chymotrypsin-like serine proteases (SPs) are one of the best-studied family of enzymes with roles in a wide range of physiological processes, including digestion, blood coagulation, fibrinolysis and humoral immunity. Extracellular SPs can form cascades, in which one protease activates the zymogen of the next...

journal_title：Biochimie

authors： Veillard F,Troxler L,Reichhart JM

更新日期：2016-03-01 00:00:00

abstract：:The ability to measure the thermodynamic stability of proteins with precision is important for both academic and applied research. Such measurements rely on mathematical models of the protein denaturation profile, i.e. the relation between a global protein signal, corresponding to the folding states in equilibrium, an...

journal_title：Biochimie

authors： Bedouelle H

更新日期：2016-02-01 00:00:00

abstract：:Leishmania is a unicellular protozoan parasite which causes leishmaniasis, a neglected tropical disease. It possess a unique thiol metabolism comprising of several proteins among which, tryparedoxin (cTXN) and tryparedoxin peroxidase (cTXNPx), function in concert as oxidoreductases, utilizing trypanothione as a source...

journal_title：Biochimie

authors： Suman SS,Equbal A,Zaidi A,Ansari MY,Singh KP,Singh K,Purkait B,Sahoo GC,Bimal S,Das P,Ali V

更新日期：2016-02-01 00:00:00

abstract：:Intracellular cytoplasmic peptidoglycan (PG) intermediate levels were determined in Staphylococcus aureus during log-phase growth in enriched media. Levels of UDP-linked intermediates were quantitatively determined using ion pairing LC-MS/MS in negative mode, and amine intermediates were quantitatively determined ster...

journal_title：Biochimie

authors： Vemula H,Ayon NJ,Gutheil WG

更新日期：2016-02-01 00:00:00

abstract：:Unspliced human immunodeficiency virus-1 (HIV-1) mRNA is capped and therefore can be translated via conventional scanning mechanism. In addition, its 5' untranslated region (5'UTR) is thought to function as an internal ribosome entry site (IRES) during G2/M-phase of cell cycle or when cap-dependent translation is inhi...

journal_title：Biochimie

authors： Smirnova VV,Terenin IM,Khutornenko AA,Andreev DE,Dmitriev SE,Shatsky IN

更新日期：2016-02-01 00:00:00

abstract：:In 1998, the concept of "green chemistry" was established through twelve principles with the aim of improving the eco-efficiency of chemical processes and to judge, whether or not, a chemical process is sustainable. Currently, numerous processes do not obey to most of these principles (large energy usage, formation of...

journal_title：Biochimie

authors： Durand E,Lecomte J,Villeneuve P

更新日期：2016-01-01 00:00:00

abstract：:Stroke is a worldwide major cause of mortality and morbidity. Preclinical studies have identified over 1000 molecules with brain-protective properties. More than 200 clinical trials have evaluated neuroprotective candidates for ischemic stroke yet, to date almost all failed, leading to a re-analysis of treatment strat...

journal_title：Biochimie

authors： Blondeau N

更新日期：2016-01-01 00:00:00

abstract：:We previously reported that the rRNA methyltransferases RsmI and RsmH, which are responsible for cytidine dimethylation at position 1402 of 16S rRNA in the decoding center of the ribosome, contribute to Staphylococcus aureus virulence. Here we evaluated other 16S rRNA methyltransferases, including KsgA (RsmA), RsmB/F,...

journal_title：Biochimie

authors： Kyuma T,Kizaki H,Ryuno H,Sekimizu K,Kaito C

更新日期：2015-12-01 00:00:00

abstract：:A high level of subcellular compartmentalization is a hallmark of eukaryotic cells. This intricate internal organization was present already in the common ancestor of all extant eukaryotes, and the determination of the origins and early evolution of the different organelles remains largely elusive. Organellar proteome...

journal_title：Biochimie

authors： Gabaldón T,Pittis AA

更新日期：2015-12-01 00:00:00